Abstract: Rural non-point source pollution governance is an important element of ecological environmental protection，which is related to the construction of rural ecological civilization，national food security and agricultural green development，and the water tank，rice bag and vegetable basket of urban and rural residents．In order to solve the pollution problem of rural non-point source，explore the co-governance mode of multi-subject participation consisting of government，agribusinesses and farmers，evolutionary game model of local government，agribusinesses and farmers is built，the dominant advantage of local government in forcing farmers is explored to “reduce the amount at source” by taking advantage of the leading role of agribusinesses in the industrial chain to link up with the market and farmers．Specifically，local governments act on agribusinesses through administrative orders and financial subsides to supervise and support the development of agribusinesses and gain policy benefits from the central government. Agribusinesses，as government agents，supervise the green production behavior of farmers，after supervising and testing the quality of agricultural products，adopt a graded purchase system or give farmers green premium dividends to take care of farmers，thus driving farmers to provide high-quality green products according to the contract signed between the two parties．At the same time，agricultural enterprises also realize the green supply chain revenue．

Key words: agricultural non-point source pollution; government-agricultural enterprise-farmer collaboration; evolutionary game